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Image Search Results
Journal: medRxiv
Article Title: Systems biological assessment of the temporal dynamics of immunity to a viral infection in the first weeks and months of life
doi: 10.1101/2023.01.28.23285133
Figure Lengend Snippet: Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron spike-BV421+. B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Article Snippet: The following antibodies were used: IgD PE (BD Biosciences, 555779), IgM PerCP-Cy5.5 (BioLegend, 314512), CD20 APC-H7 (BD Biosciences, 560734), CD27 PE-Cy7 (BioLegend, 302838), CD14 PE/Dazzle™ 594 (BioLegend, 301852), CD16 BV605 (BioLegend, 302040), IgG BV650 (BD Biosciences, 740596), CD3 BUV737 (BD Biosciences, 612750) and Alexa Fluor 488-labeled Wuhan spike (SinoBiological, 40589-V27B-B), and
Techniques: Staining, FACS, Flow Cytometry, Infection